MSD: How Do Researchers Find Efficient Ways To Analyze Multiple Molecules?

Meso Scale Diagnostics offers a spectrum of immunoassays to detect and quantify analytes in complex biological matrices. With an arsenal of over 400 immunoassay kits and assay development services, Meso Scale Discovery has enabled researchers to accurately determine proteins, cytokines, and other biomarkers in different study samples. Meso Scale Discovery (MSD) platform provides high-quality data in less time and with minimal effort. Hence, MSD Mesoscale technology has become the primary detection technique in several biomedical, pharmaceutical, and biotech companies.

MSD analysis employs next-gen electrochemiluminescence technology to detect analytes in singleplex and multiplex formats. It combines electrochemiluminescence detection with pattern array technology to provide rapid and highly dense information through organization, miniaturization, and parallel processing. MSD product line comprises diverse immunoassay kits and assay development reagents and plates, making them crucial for each phase of drug development. Let us now focus on ways researchers use MSD assays to analyze multiple molecules.

MSD technology

MSD technology is based on three fundamental components; electrochemiluminescence detection, multi-array technology, and MSD instruments. Let’s dive deep into each of these aspects in more detail.

Electrochemiluminescence detection enhances sensitivity, convenience, and dynamic range. This system can generate high-quality and reliable results in various study matrices such as blood, serum, plasma, and urine.

Multi-array technology integrates electrochemiluminescence detection and multiple arrays to provide a rapid and high influx of information. This feature is coupled with multi-spot plates that allow the quantification of numerous analytes in small sample volumes.

MSD has two robust instruments: MESO SECTOR S 600M and MESO QuickPlex SQ 120MM. Both these technologies are available for basic and applied biomedical research. Besides, both these platforms work through the Discovery Workbench software to efficiently acquire and analyze study samples. However, assay development and validation will always be crucial for reliable analytical method transfer in pharma and biomedical sciences.

Multi-spot plates

Multi-spot plates have arrays within each well. Each well can have up to 10 spots,  increasing assay throughput and multiplexing capacities. Besides, MSD assay comes in 96-well and 384-well plates.

Multi-spot plates provide ultra-low detection, use minimal samples, provide up to five logs of dynamic range, and handle complex matrices. These advantages ensure that native levels of analytes can be determined easily without multiple dilutions. Besides, MSD assays are streamlined to reduce workflows without compromising assay data. 

Measurement of biomarkers in complex biological matrices

Clinical samples are innately complex. Complex study matrices, limited biological samples, and a wide range of analyte concentrations further increase the challenges for conventional immunoassays. However, MSD assays overcome all the above obstacles. They provide enhanced sensitivity, save samples, expand the assay range, and handle complex biological matrices. 

Also Read: An Insight Into Sandwich ELISA Protocols

The highly adaptable feature lets MSD assay quantify analytes in all types of study matrices. A broad dynamic range means biomarkers in both disease and control samples don’t require multiple dilutions. And multiplexing capacities can determine several biomarkers in a single assay volume. Moreover, they are ideal for regulatory environments as they support 21 CFR Part 11 and GLP requirements.

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